PLD6 antibody (AA 39-238)

Catalog No. ABIN8022212

This Rabbit Polyclonal antibody specifically detects PLD6 in ELISA, WB, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for PLD6 antibody (AA 39-238) (ABIN8022212)

Target: PLD6 (Phospholipase D Family, Member 6 (PLD6))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLD6 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-PLD6 Antibody

Binding Specificity: AA 39-238

Purpose: Anti-PLD6 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PLD6 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PLD6 recombinant protein (Position: R39-L238).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PLD6

Target: PLD6 (Phospholipase D Family, Member 6 (PLD6))

Alternative Name: PLD6

Background:

Background: PLD6 (phospholipase D family, member 6) is a protein-coding gene. Among its related super-pathways are choline biosynthesis III and Glycerophospholipid biosynthesis. GO annotations related to this gene include cardiolipin hydrolase activity and protein homodimerization activity. Regulates mitochondrial shape through facilitating mitochondrial fusion. During spermatogenesis, plays a critical role in PIWI-interacting RNA (piRNA) biogenesis (By similarity). piRNAs provide essential protection against the activity of mobile genetic elements. piRNA-mediated transposon silencing is thus critical for maintaining genome stability, in particular in germline cells when transposons are mobilized as a consequence of wide-spread genomic demethylation. Has been shown to be a backbone-non-specific, single strand-specific nuclease, cleaving either RNA or DNA substrates with similar affinity (By similarity). Produces 5' phosphate and 3' hydroxyl termini, suggesting it could ly participate in the processing of primary piRNA transcripts (By similarity). Has been proposed to act as a cardiolipin hydrolase to generate phosphatidic acid at mitochondrial surface. Although it cannot be excluded that it can act as a phospholipase in some circumstances, it should be noted that cardiolipin hydrolase activity is either undetectable in vitro, or very low (PubMed:21397848). In addition, cardiolipin is almost exclusively found on the inner mitochondrial membrane, while PLD6 localizes to the outer mitochondrial membrane, facing the cytosol.

Gene Full Name: phospholipase D family member 6

Molecular Weight: 28 kDa

Gene ID: 201164

UniProt: Q8N2A8

Pathways: Ribonucleoprotein Complex Subunit Organization