PPAT antibody (AA 77-359) (HRP)

Catalog No. ABIN8022351

The Rabbit Polyclonal anti-PPAT antibody is suitable to detect PPAT in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for PPAT antibody (AA 77-359) (HRP) (ABIN8022351)

Target: PPAT (Phosphoribosyl Pyrophosphate Amidotransferase (PPAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPAT antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-PPAT Antibody (HRP)

Binding Specificity: AA 77-359

Purpose: Anti-PPAT Antibody HRP Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human PPAT shares 92.2%,94.3% amino acid (aa) sequence identity with mouse,rat PPAT,respectively.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PPAT recombinant protein (Position: D77-Q359). Human PPAT shares 92.2% and 94.3% amino acid (aa) sequence identity with mouse and rat PPAT, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPAT

Target: PPAT (Phosphoribosyl Pyrophosphate Amidotransferase (PPAT))

Alternative Name: PPAT

Background:

Background: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.

Gene Full Name: phosphoribosyl pyrophosphate amidotransferase

Gene ID: 5471

UniProt: Q06203