PPAT antibody (AA 77-359)

Catalog No. ABIN8022355

The Rabbit Polyclonal anti-PPAT antibody (ABIN8022355) specifically detects PPAT in WB, ELISA, FACS, ICC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PPAT antibody (AA 77-359) (ABIN8022355)

Target: PPAT (Phosphoribosyl Pyrophosphate Amidotransferase (PPAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPAT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-PPAT Antibody

Binding Specificity: AA 77-359

Purpose: Anti-PPAT Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human PPAT shares 92.2%,94.3% amino acid (aa) sequence identity with mouse,rat PPAT,respectively.

Characteristics: Anti-PPAT Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PPAT recombinant protein (Position: D77-Q359). Human PPAT shares 92.2% and 94.3% amino acid (aa) sequence identity with mouse and rat PPAT, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PPAT

Target: PPAT (Phosphoribosyl Pyrophosphate Amidotransferase (PPAT))

Alternative Name: PPAT

Background:

Background: The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. It is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosythetic pathway. This gene and PAICS/AIRC gene, a bifunctional enzyme catalyzing steps six and seven of this pathway, are located in close proximity on chromosome 4, and divergently transcribed from an intergenic region.

Gene Full Name: phosphoribosyl pyrophosphate amidotransferase

Molecular Weight: 57 kDa

Gene ID: 5471

UniProt: Q06203