PTTG1 antibody (C-Term) (Cy3)

Catalog No. ABIN8022646

The Cy3-conjugated Rabbit Polyclonal anti-PTTG1 antibody (ABIN8022646) specifically detects PTTG1 in FACS. The antibody is reactive with Human samples.

Quick Overview for PTTG1 antibody (C-Term) (Cy3) (ABIN8022646)

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PTTG1 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-PTTG1 Antibody (Cy3)

Binding Specificity: C-Term

Purpose: Anti-Securin/PTTG1 Antibody Cy3 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat sequence by four amino acids,and from the related mouse sequence by five amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Securin, different from the related rat sequence by four amino acids, and from the related mouse sequence by five amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for PTTG1

Target: PTTG1 (Pituitary Tumor-Transforming 1 (PTTG1))

Alternative Name: PTTG1

Background:

Background: Securin, also known as pituitary tumor-transforming 1 or EAP1 is a protein that in humans is encoded by the PTTG1 gene. PTTG1 gene is mapped to 5q33.3. The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.

Gene Full Name: PTTG1 regulator of sister chromatid separation, securin

Sequence Similarities: Belongs to the securin family.

Gene ID: 9232

UniProt: O95997