POLR1B antibody (AA 18-1135) (APC)

Catalog No. ABIN8024227

The APC-conjugated Rabbit Polyclonal anti-POLR1B antibody (ABIN8024227) specifically detects POLR1B in WB, ELISA, IHC, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for POLR1B antibody (AA 18-1135) (APC) (ABIN8024227)

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1B antibody is conjugated to APC

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-POLR1B Antibody (APC)

Binding Specificity: AA 18-1135

Purpose: Anti-POLR1B Antibody APC Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human POLR1B recombinant protein (Position: H18-V1135).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for POLR1B

Target: POLR1B (Polymerase (RNA) I Polypeptide B, 128kDa (POLR1B))

Alternative Name: POLR1B

Background:

Background: DNA-ed RNA polymerase I subunit RPA2 is an enzyme that in humans is encoded by the POLR1B gene. Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2.

Gene Full Name: RNA polymerase I subunit B

Gene ID: 84172

UniProt: Q9H9Y6