POU2AF1 antibody (C-Term) (Cy3)

Catalog No. ABIN8024817

The Rabbit Polyclonal anti-POU2AF1 antibody is suitable to detect POU2AF1 in samples from Rat and Mouse. It has been validated for FACS.

Quick Overview for POU2AF1 antibody (C-Term) (Cy3) (ABIN8024817)

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU2AF1 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-POU2AF1 Antibody (Cy3)

Binding Specificity: C-Term

Purpose: Anti-BOB1/POU2AF1 Antibody Cy3 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat sequence by one amino acid,and from human sequence by four amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1, different from the related rat sequence by one amino acid, and from human sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for POU2AF1

Target: POU2AF1 (POU Class 2 Associating Factor 1 (POU2AF1))

Alternative Name: POU2AF1

Background:

Background: POU2AF1 (POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V (D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by ly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.

Gene Full Name: POU domain, class 2, associating factor 1

Gene ID: 18985

UniProt: Q64693