PHTF1 antibody (AA 188-430) (Fluoro488)

Catalog No. ABIN8029124

This Rabbit Polyclonal antibody specifically detects PHTF1 in FACS. It exhibits reactivity toward Human.

Quick Overview for PHTF1 antibody (AA 188-430) (Fluoro488) (ABIN8029124)

Target: PHTF1 (Putative Homeodomain Transcription Factor 1 (PHTF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHTF1 antibody is conjugated to Fluoro488

Application: Flow Cytometry (FACS)

Product Details anti-PHTF1 Antibody (Fluoro488)

Binding Specificity: AA 188-430

Purpose: Anti-PHTF1 Antibody Fluoro488 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PHTF1 recombinant protein (Position: I188-Q430).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for PHTF1

Target: PHTF1 (Putative Homeodomain Transcription Factor 1 (PHTF1))

Alternative Name: PHTF1

Background:

Background: HTF1 (putative homeodomain transcription factor 1), also known as PHTF, is a potential transcription regulator. It is a ubiquitously expressed integral, multipass membrane protein with predominant expression in testis. PHTF1 is associated with the ER (endoplasmic reticulum) and contains one bHLH (basic helix-loop-helix) domain. It is present in the cell during meiosis and spermiogenesis but, by the end of spermiogenesis, is released from the mature cell within the residual bodies. This implies that PHTF1 may play a role in the spermatozoa maturation process. In addition, PHTF1 is believed to interact with FEM1B and may be responsible for recruiting FEM1B to the surface of the ER membrane. This suggests that PHTF1 acts as a sequestering or anchoring protein for FEM1B. Two PHTF1 isoforms exist due to alternate splicing events. Isoform 2 is the shorter form and lacks the amino acid residues 648 to 762.

Gene Full Name: putative homeodomain transcription factor 1

Gene ID: 10745

UniProt: Q9UMS5