PHTF1 antibody (AA 188-430)

Catalog No. ABIN8029132

The Rabbit Polyclonal anti-PHTF1 antibody (ABIN8029132) specifically detects PHTF1 in WB, FACS and ELISA. The antibody is reactive with Human samples.

Quick Overview for PHTF1 antibody (AA 188-430) (ABIN8029132)

Target: PHTF1 (Putative Homeodomain Transcription Factor 1 (PHTF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHTF1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Grade: Carrier-free

Product Details anti-PHTF1 Antibody

Binding Specificity: AA 188-430

Purpose: Anti-PHTF1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PHTF1 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PHTF1 recombinant protein (Position: I188-Q430).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PHTF1

Target: PHTF1 (Putative Homeodomain Transcription Factor 1 (PHTF1))

Alternative Name: PHTF1

Background:

Background: HTF1 (putative homeodomain transcription factor 1), also known as PHTF, is a potential transcription regulator. It is a ubiquitously expressed integral, multipass membrane protein with predominant expression in testis. PHTF1 is associated with the ER (endoplasmic reticulum) and contains one bHLH (basic helix-loop-helix) domain. It is present in the cell during meiosis and spermiogenesis but, by the end of spermiogenesis, is released from the mature cell within the residual bodies. This implies that PHTF1 may play a role in the spermatozoa maturation process. In addition, PHTF1 is believed to interact with FEM1B and may be responsible for recruiting FEM1B to the surface of the ER membrane. This suggests that PHTF1 acts as a sequestering or anchoring protein for FEM1B. Two PHTF1 isoforms exist due to alternate splicing events. Isoform 2 is the shorter form and lacks the amino acid residues 648 to 762.

Gene Full Name: putative homeodomain transcription factor 1

Molecular Weight: 100 kDa

Gene ID: 10745

UniProt: Q9UMS5