PDXK antibody (AA 13-280)

Catalog No. ABIN8029242

The Rabbit Polyclonal anti-PDXK antibody is suitable to detect PDXK in samples from Human, Mouse and Rat. It has been validated for WB, IHC, ELISA and FACS.

Quick Overview for PDXK antibody (AA 13-280) (ABIN8029242)

Target: PDXK (Pyridoxal Kinase (PDXK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDXK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-PDXK Antibody

Binding Specificity: AA 13-280

Purpose: Anti-PDXK Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human PDXK shares 85.4%,85.1% amino acid (aa) sequence identity with mouse,rat PDXK,respectively.

Characteristics: Anti-PDXK Antibody. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDXK recombinant protein (Position: H13-E280). Human PDXK shares 85.4% and 85.1% amino acid (aa) sequence identity with mouse and rat PDXK, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for PDXK

Target: PDXK (Pyridoxal Kinase (PDXK))

Alternative Name: PDXK

Background:

Background: Pyridoxal kinase is an enzyme that in humans is encoded by the PDXK gene. The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Gene Full Name: pyridoxal kinase

Molecular Weight: 35 kDa

Gene ID: 8566

UniProt: O00764