PKM antibody (N-Term) (FITC)

Catalog No. ABIN8029424

This Mouse Monoclonal antibody specifically detects PKM in FACS. It exhibits reactivity toward Human.

Quick Overview for PKM antibody (N-Term) (FITC) (ABIN8029424)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKM antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 11I4C3

Product Details anti-PKM Antibody (FITC)

Binding Specificity: N-Term

Purpose: Anti-PKM2 Antibody (monoclonal, 11I4C3) FITC Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by five amino acids,and from the related rat sequence by four amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2, different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Alternative Name: PKM

Background:

Background: PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds ly and selectively to tyrosine-phosphorylated peptides.

Gene Full Name: pyruvate kinase M1/2

Gene ID: 5315

UniProt: P14618

Pathways: Warburg Effect