HMGCR antibody (pSer872)

Catalog No. ABIN753283

This Rabbit Polyclonal antibody specifically detects HMGCR in WB, ELISA, FACS, IF (cc), IF (p), IHC (fro) and IHC (p). It exhibits reactivity toward Human, Rat and Mouse and has been mentioned in 2+ publications.

Quick Overview for HMGCR antibody (pSer872) (ABIN753283)

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HMGCR Antibody

Binding Specificity: pSer872

Specificity: This phosphorylation site is homologous to that of Ser871 in Mouse and Rat.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human HMGCR around the phosphorylation site of Ser872

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HMGCR antibody (ABIN753283)

Zanoni, Aiello, Arnoldi, Lammi: "Hempseed Peptides Exert Hypocholesterolemic Effects with a Statin-Like Mechanism." in: Journal of agricultural and food chemistry, Vol. 65, Issue 40, pp. 8829-8838, (2017) (PubMed).

Yang, Ouyang, Chen, Pang, Dong, Yang, Liu, Peng, Wang, Zhang, Ren: "HMG-CoA reductase is negatively associated with PCV2 infection and PCV2-induced apoptotic cell death." in: The Journal of general virology, Vol. 95, Issue Pt 6, pp. 1330-7, (2014) (PubMed).

Target Details for HMGCR

Target: HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))

Alternative Name: HMGCR

Background:

Synonyms: LDLCQ3, 3-hydroxy-3-methylglutaryl-coenzyme A reductase, HMG-CoA reductase, HMGCR, phospho HMGCR, p-HMGCR

Background: Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins.

Gene ID: 3156

UniProt: P04035

Pathways: AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha