RHOT2 antibody (AA 321-615)

Catalog No. ABIN8030715

The Rabbit Polyclonal anti-RHOT2 antibody is suitable to detect RHOT2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, FACS, ICC, IP and IF.

Quick Overview for RHOT2 antibody (AA 321-615) (ABIN8030715)

Target: RHOT2 (Ras Homolog Gene Family, Member T2 (RHOT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-RHOT2 Antibody

Binding Specificity: AA 321-615

Purpose: Anti-MIRO2/RHOT2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human MIRO2,RHOT2 shares 78.5%,76.3% amino acid (aa) sequence identity with mouse,rat MIRO2,RHOT2,respectively.

Characteristics: Anti-MIRO2/RHOT2 Antibody. Tested in ELISA, Flow Cytometry, IP, IF, IHC, ICC, WB applications. This antibody reacts with Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MIRO2/RHOT2 recombinant protein (Position: D321-V615). Human MIRO2/RHOT2 shares 78.5% and 76.3% amino acid (aa) sequence identity with mouse and rat MIRO2/RHOT2, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RHOT2

Target: RHOT2 (Ras Homolog Gene Family, Member T2 (RHOT2))

Alternative Name: RHOT2

Background:

Background: This gene encodes a member of the Rho family of GTPases. The encoded protein is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics.

Gene Full Name: ras homolog family member T2

Molecular Weight: 80 kDa

Gene ID: 89941

UniProt: Q8IXI1