ARHGEF12 antibody (AA 10-1426)

Catalog No. ABIN8032160

This Rabbit Polyclonal antibody specifically detects ARHGEF12 in ELISA, WB, IHC, FACS, IF, IP and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ARHGEF12 antibody (AA 10-1426) (ABIN8032160)

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-ARHGEF12 Antibody

Binding Specificity: AA 10-1426

Purpose: Anti-LARG/ARHGEF12 Antibody

Predicted Reactivity: Human ARHGEF12 shares 89.9% amino acid (aa) sequence identity with mouse ARHGEF12.

Characteristics: Anti-LARG/ARHGEF12 Antibody. Tested in WB, IHC, ICC, IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human LARG/ARHGEF12 recombinant protein (Position: D10-E1426). Human ARHGEF12 shares 89.9% amino acid (aa) sequence identity with mouse ARHGEF12.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ARHGEF12

Target: ARHGEF12 (rho Guanine Nucleotide Exchange Factor (GEF) 12 (ARHGEF12))

Alternative Name: ARHGEF12

Background:

Background: Rho guanine nucleotide exchange factor 12 is a protein that in humans is encoded by the ARHGEF12 gene. Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: Rho guanine nucleotide exchange factor 12

Molecular Weight: 220 kDa

Gene ID: 23365

UniProt: Q9NZN5

Pathways: Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling