Rhotekin antibody (AA 255-554)

Catalog No. ABIN8032292

This Rabbit Polyclonal antibody specifically detects Rhotekin in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for Rhotekin antibody (AA 255-554) (ABIN8032292)

Target: Rhotekin (RTKN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Rhotekin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-Rhotekin Antibody

Binding Specificity: AA 255-554

Purpose: Anti-RTKN Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RTKN Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RTKN recombinant protein (Position: R255-Q554).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Rhotekin

Target: Rhotekin (RTKN)

Alternative Name: RTKN

Background:

Background: Rhotekin is a protein that in humans is encoded by the RTKN gene. This gene encodes a scaffold protein that interacts with GTP-bound Rho proteins. Binding of this protein inhibits the GTPase activity of Rho proteins. This protein may interfere with the conversion of active, GTP-bound Rho to the inactive GDP-bound form by RhoGAP. Rho proteins regulate many important cellular processes, including cytokinesis, transcription, smooth muscle contraction, cell growth and transformation. Dysregulation of the Rho signal transduction pathway has been implicated in many forms of cancer. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Full Name: rhotekin

Molecular Weight: 70 kDa

Gene ID: 6242

UniProt: Q9BST9