Ribophorin II antibody (AA 64-174)

Catalog No. ABIN8032491

The Rabbit Polyclonal anti-Ribophorin II antibody is suitable to detect Ribophorin II in samples from Human. It has been validated for WB, IHC, ELISA, FACS, IF and ICC.

Quick Overview for Ribophorin II antibody (AA 64-174) (ABIN8032491)

Target: Ribophorin II (RPN2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ribophorin II antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-Ribophorin II Antibody

Binding Specificity: AA 64-174

Purpose: Anti-RPN2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RPN2 Antibody. Tested in ELISA, Flow Cytometry, IF, ICC, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RPN2 recombinant protein (Position: Q64-Q174).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Ribophorin II

Target: Ribophorin II (RPN2)

Alternative Name: RPN2

Background:

Background: Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 2, also called ribophorin is an enzyme that in humans is encoded by the RPN2 gene. This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: ribophorin II

Molecular Weight: 69 kDa

Gene ID: 6185

UniProt: P04844