RPL27 antibody (AA 1-108)

Catalog No. ABIN8032656

The Rabbit Polyclonal anti-RPL27 antibody is suitable to detect RPL27 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, FACS and IP.

Quick Overview for RPL27 antibody (AA 1-108) (ABIN8032656)

Target: RPL27 (Ribosomal Protein L27 (RPL27))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP)

Grade: Carrier-free

Product Details anti-RPL27 Antibody

Binding Specificity: AA 1-108

Purpose: Anti-RPL27 Antibody

Predicted Reactivity: Human RPL27 shares 100% amino acid (aa) sequence identity with both mouse,rat RPL27.

Characteristics: Anti-RPL27 Antibody. Tested in WB, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RPL27 recombinant protein (Position: M1-R108). Human RPL27 shares 100% amino acid (aa) sequence identity with both mouse and rat RPL27.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RPL27

Target: RPL27 (Ribosomal Protein L27 (RPL27))

Alternative Name: RPL27

Background:

Background: 60S ribosomal protein L27 is a protein that in humans is encoded by the RPL27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Gene Full Name: ribosomal protein L27

Molecular Weight: 16 kDa

Gene ID: 6155

UniProt: P61353