RNF20 antibody (AA 123-389)

Catalog No. ABIN8033261

This Mouse Monoclonal antibody specifically detects RNF20 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RNF20 antibody (AA 123-389) (ABIN8033261)

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This RNF20 antibody is un-conjugated

Application: Western Blotting (WB)

Grade: Carrier-free

Clone: 3C6E2

Product Details anti-RNF20 Antibody

Binding Specificity: AA 123-389

Purpose: Anti-RNF20 Antibody (monoclonal, 3C6E2)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RNF20 Antibody.(monoclonal, 3C6E2)-4. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RNF20 recombinant protein (Position: E123-S389).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RNF20

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Alternative Name: RNF20

Background:

Background: E3 ubiquitin-protein ligase BRE1A is an enzyme that in humans is encoded by the RNF20 gene. The protein encoded by this gene shares similarity with BRE1 of S. cerevisiae. The protein encoded by this human gene is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. This protein acts as a putative tumor suppressor and positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. In contrast, this protein also suppresses the expression of several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor. This gene selectively suppresses the expression of some genes by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS).

Gene Full Name: ring finger protein 20

Molecular Weight: 114 kDa

Gene ID: 56254

UniProt: Q5VTR2