RNF220 antibody (AA 1-566)

Catalog No. ABIN8033294

This Rabbit Polyclonal antibody specifically detects RNF220 in ELISA, WB, FACS, IF, IHC and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RNF220 antibody (AA 1-566) (ABIN8033294)

Target: RNF220 (Ring Finger Protein 220 (RNF220))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF220 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-RNF220 Antibody

Binding Specificity: AA 1-566

Purpose: Anti-RNF220 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human RNF220 shares 97.9% amino acid (aa) sequence identity with mouse RNF220.

Characteristics: Anti-RNF220 Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RNF220 recombinant protein (Position: M1-L566). Human RNF220 shares 97.9% amino acid (aa) sequence identity with mouse RNF220.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for RNF220

Target: RNF220 (Ring Finger Protein 220 (RNF220))

Alternative Name: RNF220

Background:

Background: Enables beta-catenin binding activity. Involved in positive regulation of canonical Wnt signaling pathway. Acts upstream of or within positive regulation of DNA-binding transcription factor activity and protein monoubiquitination. Located in nuclear lamina. Part of protein-containing complex. Implicated in hypomyelinating leukodystrophy 23.

Gene Full Name: ring finger protein 220

Molecular Weight: 63 kDa

Gene ID: 55182

UniProt: Q5VTB9