RNF8 antibody (AA 3-488)

Catalog No. ABIN8033371

The Rabbit Polyclonal anti-RNF8 antibody (ABIN8033371) specifically detects RNF8 in WB, ELISA and FACS. The antibody is reactive with Mouse and Rat samples.

Quick Overview for RNF8 antibody (AA 3-488) (ABIN8033371)

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-RNF8 Antibody

Binding Specificity: AA 3-488

Purpose: Anti-RNF8 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RNF8 Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived mouse RNF8 recombinant protein (Position: E3-S488).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RNF8

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Alternative Name: Rnf8

Background:

Background: E3 ubiquitin-protein ligase RNF8 is an enzyme that in humans is encoded by the RNF8 gene. The protein encoded by this gene contains a RING finger motif and an FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. This protein is also known to play a role in the DNA damage response and depletion of this protein causes cell growth inhibition and cell cycle arrest. Alternative splicing results in multiple transcript variants.

Gene Full Name: ring finger protein 8

Molecular Weight: 56 kDa

Gene ID: 58230

UniProt: Q8VC56

Pathways: Production of Molecular Mediator of Immune Response