S100A9 antibody (AA 2-113)

Catalog No. ABIN8034370

The Rabbit Polyclonal anti-S100A9 antibody (ABIN8034370) specifically detects S100A9 in WB, IHC, ELISA, ICC, IF and FACS. The antibody is reactive with Mouse and Rat samples.

Quick Overview for S100A9 antibody (AA 2-113) (ABIN8034370)

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-S100A9 Antibody

Binding Specificity: AA 2-113

Purpose: Anti-S100A9 Antibody

Specificity: Cross reactivity with recombinant human S100A9 is observed.

Cross-Reactivity (Details): Cross-reactivity with recombinant human S100A9 is observed.

Predicted Reactivity: coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8%,78.6% amino acid (aa) sequence identity with human,rat S100A9,respectively.

Characteristics: Anti-S100A9 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived mouse S100A9 recombinant protein (Position: A2-K113). Mouse S100A9 shares 59.8% and 78.6% amino acid (aa) sequence identity with human and rat S100A9, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for S100A9

Target: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Alternative Name: S100a9

Background:

Background: S100 calcium-binding protein A9 (S100A9), also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B, is a protein that in humans is encoded by the S100A9 gene. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. And S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase.

Gene Full Name: S100 calcium binding protein A9 (calgranulin B)

Molecular Weight: 13-15 kDa

Gene ID: 20202

UniProt: P31725

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins