SPARC antibody (AA 107-303) (HRP)

Catalog No. ABIN8035170

The Rabbit Polyclonal anti-SPARC antibody is suitable to detect SPARC in samples from Human, Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for SPARC antibody (AA 107-303) (HRP) (ABIN8035170)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPARC antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-SPARC Antibody (HRP)

Binding Specificity: AA 107-303

Purpose: Anti-SPARC Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: Human SPARC shares 97% amino acid (aa) sequence identity with both mouse,rat SPARC.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPARC recombinant protein (Position: E107-I303). Human SPARC shares 97% amino acid (aa) sequence identity with both mouse and rat SPARC.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC

Background:

Background: SPARC, secreted protein acidic and rich in cysteine, also known as Osteonectin is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Furthermore, SPARC is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. SPARC also shows affinity for collagen in addition to bone mineral calcium. A correlation between SPARC over expression and ampullary cancers and chronic pancreatitis has been found.

Gene Full Name: secreted protein acidic and cysteine rich

Gene ID: 6678

UniProt: P09486

Pathways: Autophagy