SCLY antibody (AA 157-445)

Catalog No. ABIN8035438

The Rabbit Polyclonal anti-SCLY antibody (ABIN8035438) specifically detects SCLY in WB, ELISA, FACS and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for SCLY antibody (AA 157-445) (ABIN8035438)

Target: SCLY (Selenocysteine Lyase (SCLY))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCLY antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-SCLY Antibody

Binding Specificity: AA 157-445

Purpose: Anti-SCLY Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SCLY Antibody. Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SCLY recombinant protein (Position: E157-A445).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SCLY

Target: SCLY (Selenocysteine Lyase (SCLY))

Alternative Name: SCLY

Background:

Background: SCLY(Selenocysteine Lyase), is an enzyme that catalyzes the chemical reaction. This enzyme belongs to the family of lyases, specifically the class of carbon-sulfur lyases. The International Radiation Hybrid Mapping Consortium mapped the human SCLY gene to chromosome 2. Mihara et al.(2000) showed that mouse Scly catalyzed the conversion of L-selenocysteine to L-alanine. Scly activity required pyridoxal 5-prime phosphate, was specific to L-selenocysteine, and showed maximum reactivity at pH 9.0.

Gene Full Name: selenocysteine lyase

Molecular Weight: 48 kDa

Gene ID: 51540

UniProt: Q96I15

Pathways: Stem Cell Maintenance