SHMT2 antibody (AA 9-504)

Catalog No. ABIN8035857

The Rabbit Polyclonal anti-SHMT2 antibody is suitable to detect SHMT2 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, ELISA, FACS, IP and ICC.

Quick Overview for SHMT2 antibody (AA 9-504) (ABIN8035857)

Target: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHMT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-SHMT2 Antibody

Binding Specificity: AA 9-504

Purpose: Anti-SHMT2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human SHMT2 shares 95.1% amino acid (aa) sequence identity with mouse SHMT2.

Characteristics: Anti-SHMT2 Antibody. Tested in WB, IHC, IF, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SHMT2 recombinant protein (Position: A9-H504). Human SHMT2 shares 95.1% amino acid (aa) sequence identity with mouse SHMT2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunofluorescence, 5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SHMT2

Target: SHMT2 (serine Hydroxymethyltransferase 2 (Mitochondrial) (SHMT2))

Alternative Name: SHMT2

Background:

Background: This gene encodes the mitochondrial form of a pyridoxal phosphate-dependent enzyme that catalyzes the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. The encoded product is primarily responsible for glycine synthesis. The activity of the encoded protein has been suggested to be the primary source of intracellular glycine. The gene which encodes the cytosolic form of this enzyme is located on chromosome 17. Alternative splicing results in multiple transcript variants.

Gene Full Name: serine hydroxymethyltransferase 2

Molecular Weight: 56 kDa

Gene ID: 6472

UniProt: P34897