SHARPIN antibody (AA 25-325)

Catalog No. ABIN8036987

This Rabbit Polyclonal antibody specifically detects SHARPIN in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SHARPIN antibody (AA 25-325) (ABIN8036987)

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHARPIN antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-SHARPIN Antibody

Binding Specificity: AA 25-325

Purpose: Anti-SHARPIN Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human SHARPIN shares 72.3%,72.6% amino acid (aa) sequence identity with mouse,rat SHARPIN,respectively.

Characteristics: Anti-SHARPIN Antibody. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SHARPIN recombinant protein (Position: H25-R325). Human SHARPIN shares 72.3% and 72.6% amino acid (aa) sequence identity with mouse and rat SHARPIN, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SHARPIN

Target: SHARPIN (SHANK-Associated RH Domain Interacting Protein (SHARPIN))

Alternative Name: SHARPIN

Background:

Background: Enables polyubiquitin modification-dependent protein binding activity. Involved in defense response to bacterium, protein linear polyubiquitination, and regulation of signal transduction. Located in cytosol. Part of LUBAC complex.

Gene Full Name: SHANK associated RH domain interactor

Molecular Weight: 40 kDa

Gene ID: 81858

UniProt: Q9H0F6