SSH1 antibody (AA 489-921)

Catalog No. ABIN8037970

The Rabbit Polyclonal anti-SSH1 antibody (ABIN8037970) specifically detects SSH1 in WB, ELISA, FACS, IF and ICC. The antibody is reactive with Human samples.

Quick Overview for SSH1 antibody (AA 489-921) (ABIN8037970)

Target: SSH1 (Slingshot Homolog 1 (SSH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SSH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-SSH1 Antibody

Binding Specificity: AA 489-921

Purpose: Anti-SSH1 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human SSH1 shares 66.9% amino acid (aa) sequence identity with mouse SSH1.

Characteristics: Anti-SSH1 Antibody. Tested in WB, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SSH1 recombinant protein (Position: E489-Y921). Human SSH1 shares 66.9% amino acid (aa) sequence identity with mouse SSH1.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SSH1

Target: SSH1 (Slingshot Homolog 1 (SSH1))

Alternative Name: SSH1

Background:

Background: Protein phosphatase Slingshot homolog 1 is an enzyme that in humans is encoded by the SSH1 gene. The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Gene Full Name: slingshot protein phosphatase 1

Molecular Weight: 150 kDa

Gene ID: 54434

UniProt: Q8WYL5

Pathways: CXCR4-mediated Signaling Events