SPA17 antibody (AA 1-136)

Catalog No. ABIN8041484

This Rabbit Polyclonal antibody specifically detects SPA17 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for SPA17 antibody (AA 1-136) (ABIN8041484)

Target: SPA17 (Sperm Autoantigenic Protein 17 (SPA17))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPA17 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Grade: Carrier-free

Product Details anti-SPA17 Antibody

Binding Specificity: AA 1-136

Purpose: Anti-SPA17 Antibody

Cross-Reactivity (Details): No cross reactivity with other proteins.

Predicted Reactivity: Human SPA17 shares 75.7% amino acid (aa) sequence identity with mouse SPA17.

Characteristics: Anti-SPA17 Antibody. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SPA17 recombinant protein (Position: M1-K136). Human SPA17 shares 75.7% amino acid (aa) sequence identity with mouse SPA17.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunofluorescence, 5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SPA17

Target: SPA17 (Sperm Autoantigenic Protein 17 (SPA17))

Alternative Name: SPA17

Background:

Background: Sperm surface protein Sp17 is a protein that in humans is encoded by the SPA17 gene. This gene encodes a protein present at the cell surface. The N-terminus has sequence similarity to human cAMP-dependent protein kinase A (PKA) type II alpha regulatory subunit (RIIa) while the C-terminus has an IQ calmodulin-binding motif. The central portion of the protein has carbohydrate binding motifs and likely functions in cell-cell adhesion. The protein was initially characterized by its involvement in the binding of sperm to the zona pellucida of the oocyte. Recent studies indicate that it is also involved in additional cell-cell adhesion functions such as immune cell migration and metastasis. A retrotransposed pseudogene is present on chromosome 10q22.

Gene Full Name: sperm autoantigenic protein 17

Molecular Weight: 22 kDa

Gene ID: 53340

UniProt: Q15506