Splicing Factor 1 antibody (AA 160-266) (Fluoro550)

Catalog No. ABIN8041843

The Fluoro550-conjugated Mouse Monoclonal anti-Splicing Factor 1 antibody (Clone 2F5D10) (ABIN8041843) specifically detects Splicing Factor 1 in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Splicing Factor 1 antibody (AA 160-266) (Fluoro550) (ABIN8041843)

Target: Splicing Factor 1 (SF1)

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Splicing Factor 1 antibody is conjugated to Fluoro550

Application: Flow Cytometry (FACS)

Clone: 2F5D10

Product Details anti-Splicing Factor 1 Antibody (Fluoro550)

Binding Specificity: AA 160-266

Purpose: Anti-splicing factor 1 Antibody (monoclonal, 2F5D10) Fluoro550 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human splicing factor 1 recombinant protein (Position: R160-Q266).

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF1

Background:

Background: Splicing factor 1 also known as zinc finger protein 162 (ZFM162) is a protein that in humans is encoded by the SF1 gene. This gene encodes a nuclear pre-mRNA splicing factor. The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly. It also plays a role in nuclear pre-mRNA retention and transcriptional repression. The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain. Alternative splicing results in multiple transcript variants.

Gene Full Name: splicing factor 1

Gene ID: 7536

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location