SFPQ antibody (Middle Region)

Catalog No. ABIN8041950

The Rabbit Polyclonal anti-SFPQ antibody is suitable to detect SFPQ in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF, FACS and ICC.

Quick Overview for SFPQ antibody (Middle Region) (ABIN8041950)

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SFPQ antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Grade: Carrier-free

Product Details anti-SFPQ Antibody

Binding Specificity: Middle Region

Purpose: Anti-SFPQ Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse sequences.

Characteristics: Anti-SFPQ Antibody. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SFPQ, identical to the related mouse sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for SFPQ

Target: SFPQ (Splicing Factor Proline/glutamine-Ric (SFPQ))

Alternative Name: SFPQ

Background:

Background: Splicing factor, proline- and glutamine-rich is a protein that in humans is encoded by the SFPQ gene. Enables DNA binding activity, histone deacetylase binding activity, and protein homodimerization activity. Involved in several processes, including alternative mRNA splicing, via spliceosome, positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway, and regulation of transcription by RNA polymerase II. Acts upstream of or within double-strand break repair via homologous recombination. Located in chromatin, nuclear matrix, and paraspeckles.

Gene Full Name: splicing factor proline and glutamine rich

Molecular Weight: 100 kDa

Gene ID: 6421

UniProt: P23246