Spt6 antibody (AA 564-1639) (FITC)

Catalog No. ABIN8043816

The Rabbit Polyclonal anti-Spt6 antibody is suitable to detect Spt6 in samples from Human, Mouse, Rat and Monkey. It has been validated for FACS.

Quick Overview for Spt6 antibody (AA 564-1639) (FITC) (ABIN8043816)

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Spt6 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-Spt6 Antibody (FITC)

Binding Specificity: AA 564-1639

Purpose: Anti-Spt6/SUPT6H Antibody FITC Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Spt6/SUPT6H recombinant protein (Position: A564-Q1639).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Spt6

Target: Spt6 (SUPT6H) (Suppressor of Ty 6 Homolog (SUPT6H))

Alternative Name: SUPT6H

Background:

Background: Transcription elongation factor SPT6 is a protein that in humans is encoded by the SUPT6H gene. SUPT6H (suppressor of Ty6 homolog), also known as SPT6, SPT6H, Tat-CT2 (Tat-cotransactivator 2 protein) or emb-5 in C. elegans, is a 1,726 amino acid protein that is highly conserved from yeast to humans. Expressed ubiquitously, SUPT6H localizes to the nucleus and contains one SH2 domain and one S1 domain. SUPT6H participates in both DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole)-mediated transcriptional inhibition as well as the enhancement of transcriptional elongation by the RNA polymerase II (Pol II). SUPT6H interacts with the nuclear proteins SPT4 and SPT5, which comprise the DSIF (DRB-sensitivity-inducing factor) complex that binds RNA polymerase II, and ly regulates elongation. Via its C-terminus, SUPT6H can also interact with Histone H3. Due to alternative splicing events, three isoforms exist for SUPT6H. This antibody specifically recognizes the 230kd phosphorylated SUPT6H protein.

Gene Full Name: SPT6 homolog, histone chaperone and transcription elongation factor

Gene ID: 6830

UniProt: Q7KZ85

Pathways: Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response