PKC theta antibody (AA 351-450)

Catalog No. ABIN754738

This Rabbit Polyclonal antibody specifically detects PKC theta in WB, ELISA, IHC (p), IF (cc), IF (p) and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for PKC theta antibody (AA 351-450) (ABIN754738)

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC theta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PKC theta Antibody

Binding Specificity: AA 351-450

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Dog,Cow,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PKC ta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Alternative Name: PKC theta

Background:

Synonyms: PKC 0, PRKCQ, PKC0, Prkcq, PRKCT, Protein kinase C theta, Protein kinase C theta type, Protein Kinase Ctheta, KPCT_HUMAN, nPKC theta, nPKC-theta, nPKCtheta, MGC126514, MGC141919.

Background: This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Gene ID: 5588

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling