TRAC antibody (AA 1-114)

Catalog No. ABIN8045000

This Rabbit Polyclonal antibody specifically detects TRAC in ELISA, FACS, WB and IHC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TRAC antibody (AA 1-114) (ABIN8045000)

Target: TRAC (T Cell Receptor alpha Constant (TRAC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAC antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-TRAC Antibody

Binding Specificity: AA 1-114

Purpose: Anti-TCR alpha/TRAC Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TCR alpha/TRAC Antibody. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human TCR alpha recombinant protein (Position: I1-L114).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/m Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for TRAC

Target: TRAC (T Cell Receptor alpha Constant (TRAC))

Alternative Name: TRAC

Background:

Background: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor alpha and delta loci. Both the alpha and delta loci include V (variable), J (joining), and C (constant) segments and the delta locus also includes diversity (D) segments. The delta locus is situated within the alpha locus, between the alpha V and J segments. During T cell development, the delta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment, a V segment is then joined to the D-J gene. The alpha chain is synthesized by recombination joining a single V segment with a J segment. For both chains, the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Five variable segments can be used in either alpha or delta chains and are described by TRAV/DV symbols. Several V and J segments of the alpha locus are known to be incapable of encoding a protein and are considered pseudogenes.

Gene Full Name: T cell receptor alpha constant

Molecular Weight: 45 kDa

Gene ID: 28755

UniProt: P01848