T-Bet antibody (Middle Region) (HRP)

Catalog No. ABIN8045018

This Rabbit Polyclonal antibody specifically detects T-Bet in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for T-Bet antibody (Middle Region) (HRP) (ABIN8045018)

Target: T-Bet

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Bet antibody is conjugated to HRP

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-T-Bet Antibody (HRP)

Binding Specificity: Middle Region

Purpose: Anti-T-bet/Tbx21 Antibody HRP Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse,rat sequences.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human T-bet/Tbx21, identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: Western blot, Optimal dilutions should be determined by end users. Immunohistochemistry (Paraffin-embedded Section), Optimal dilutions should be determined by end users. ELISA, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for T-Bet

Target: T-Bet

Alternative Name: TBX21

Background:

Background: T-box transcription factor TBX21 is a protein that in humans is encoded by the TBX21 gene. It is mapped to 17q21.32. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.

Gene Full Name: T-box transcription factor 21

Gene ID: 30009

UniProt: Q9UL17