T-Bet antibody (C-Term) (FITC)

Catalog No. ABIN8045028

The Rabbit Polyclonal anti-T-Bet antibody is suitable to detect T-Bet in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for T-Bet antibody (C-Term) (FITC) (ABIN8045028)

Target: T-Bet

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T-Bet antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-T-Bet Antibody (FITC)

Binding Specificity: C-Term

Purpose: Anti-T-bet/Tbx21 Antibody FITC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related rat,mouse sequences by one amino acid.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human T-bet, different from the related rat and mouse sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for T-Bet

Target: T-Bet

Alternative Name: TBX21

Background:

Background: TBX21 (T-Box 21), also called TBET, is a protein that in humans is encoded by the TBX21 gene. This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. Zhang and Yang (2000) mapped the mouse Tbx21 gene to chromosome 11D in an area showing homology of synteny with human chromosome 17. Szabo et al. (2000) showed that TBX21 expression correlates with IFNG expression in Th1 and natural killer (NK) cells. Ectopic expression of TBX21 both transactivated the IFNG gene and induced endogenous IFNG production. Finotto et al. (2002) observed reduced expression of the T (H)1 transcription factor Tbet in T cells from airways of patients with asthma compared with that in T cells from airways of nonasthmatic patients, suggesting that loss of Tbet might be associated with asthma.

Gene Full Name: T-box transcription factor 21

Sequence Similarities: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.

Gene ID: 30009

UniProt: Q9UL17