SOX9 antibody (AA 121-220)

Catalog No. ABIN754963

This Rabbit Polyclonal antibody specifically detects SOX9 in WB, ELISA, FACS, IHC (p), IF (p), IF (cc) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat and Rabbit and has been mentioned in 9+ publications.

Quick Overview for SOX9 antibody (AA 121-220) (ABIN754963)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX9 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-SOX9 Antibody

Binding Specificity: AA 121-220

Cross-Reactivity: Human, Mouse, Rabbit, Rat

Predicted Reactivity: Dog,Cow,Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human SOX9

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-SOX9 antibody (ABIN754963)

Webster, Cooley-Themm, Barnett, Bach, Vainner, Webster, Linn: "Evidence of BrdU-positive retinal neurons after application of an Alpha7 nicotinic acetylcholine receptor agonist." in: Neuroscience, Vol. 346, pp. 437-446, (2017) (PubMed).

Montorsi, Guizzetti, Alecci, Caporali, Martello, Atene, Parenti, Pizzini, Zanovello, Bortoluzzi, Ferrari, Grande, Zanocco-Marani: "Loss of zfp36 expression in colorectal cancer correlates to wnt/ ß-catenin activity and enhances epithelial-to-mesenchymal transition through upregulation of zeb1, sox9 and macc1." in: Oncotarget, Vol. 7, Issue 37, pp. 59144-59157, (2016) (PubMed).

Yang, Lu, Guo: "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." in: Molecular medicine reports, Vol. 14, Issue 5, pp. 4075-4082, (2016) (PubMed).

Chang, Lin, Lin, Wang, Hsu, Yeh: "The repair of full-thickness articular cartilage defect using intra-articular administration of N-acetyl-D-glucosamine in the rabbit knee: randomized controlled trial." in: Biomedical engineering online, Vol. 14, pp. 105, (2016) (PubMed).

Wu, Zhu, Sun, Hu: "Subchondral bone changes and chondrogenic capacity of progenitor cells from subchondral bone in the collagenase-induced temporomandibular joints osteoarthritis rabbit model." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 9782-9, (2016) (PubMed).

Wang, Ju, Sun, Hou, Yang, Zhang: "Overexpression of heparan sulfate 6-O-sulfotransferase-2 enhances fibroblast growth factor-mediated chondrocyte growth and differentiation." in: International journal of molecular medicine, Vol. 36, Issue 3, pp. 825-32, (2015) (PubMed).

Papaioannou, Mirzamohammadi, Lisse, Nishimori, Wein, Kobayashi: "MicroRNA-140 provides robustness to the regulation of hypertrophic chondrocyte differentiation by the PTHrP-HDAC4 pathway." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, (2014) (PubMed).

Mi, Chen, Wu, Wang, Gao: "Study of the effect of miR‑124 and the SOX9 target gene in Hirschsprung's disease." in: Molecular medicine reports, Vol. 9, Issue 5, pp. 1839-43, (2014) (PubMed).

Target Details for SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Alternative Name: SOX9

Background:

Synonyms: CMD1, SRA1, CMPD1, Transcription factor SOX-9, SOX9

Background: Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.

Gene ID: 6662

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development