TOP3B antibody (AA 128-824)

Catalog No. ABIN8047588

The Rabbit Polyclonal anti-TOP3B antibody (ABIN8047588) specifically detects TOP3B in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TOP3B antibody (AA 128-824) (ABIN8047588)

Target: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TOP3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Carrier-free

Product Details anti-TOP3B Antibody

Binding Specificity: AA 128-824

Purpose: Anti-TOP3B Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TOP3B Antibody. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TOP3B recombinant protein (Position: E128-R824).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for TOP3B

Target: TOP3B (Topoisomerase (DNA) III beta (TOP3B))

Alternative Name: TOP3B

Background:

Background: DNA topoisomerase 3-beta-1 is an enzyme that in humans is encoded by the TOP3B gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Low expression of this gene may be related to higher survival rates in breast cancer patients. This gene has a pseudogene on chromosome 22. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Gene Full Name: DNA topoisomerase III beta

Molecular Weight: 90-97 kDa

Gene ID: 8940

UniProt: O95985