Phospholamban antibody (AA 1-35) (HRP)

Catalog No. ABIN755272

This Rabbit Polyclonal antibody specifically detects Phospholamban in ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human.

Quick Overview for Phospholamban antibody (AA 1-35) (HRP) (ABIN755272)

Target: Phospholamban (PLN)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Phospholamban Antibody (HRP)

Binding Specificity: AA 1-35

Predicted Reactivity: Human,Mouse,Rat,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human phospholamban

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: phospholamban

Background:

Synonyms: Cardiac phospholamban, CMD1P, PLB, PLN, PPLA_HUMAN.

Background: The Sarco(endo)plasmic-reticulum (SER) regulatory protein, Phospholamban (PLB), is a small, plasma membrane-associated protein found in the SER of cardiac, smooth and slow-twitch muscle. Believed to assemble into a pentamer, PLB regulates cardiac contractility and Ca2+ affinity for cardiac SER Ca2+ ATPase (SERCA2a). Non-phosphorylated PLB associates with SERCA2a, and inhibits Ca2+ reuptake into the SER. PLB activation occurs when key Serine/Threonine residues in PLB (Ser-10, Ser-16, Thr-17) are phosphorylated by numerous effectors, which include PKC, PKA, PKG, and CaM kinase. Phosphorylation of PLB causes dissociation from SERCA2a and a subsequent increase in the rate of Ca2+ reuptake into the SER, which accelerates ventricular relaxation.

Gene ID: 5350

Pathways: Negative Regulation of Transporter Activity