TPSAB1 antibody (AA 65-275) (Cy3)

Catalog No. ABIN8050279

The Cy3-conjugated Rabbit Polyclonal anti-TPSAB1 antibody (ABIN8050279) specifically detects TPSAB1 in FACS. The antibody is reactive with Human samples.

Quick Overview for TPSAB1 antibody (AA 65-275) (Cy3) (ABIN8050279)

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPSAB1 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-TPSAB1 Antibody (Cy3)

Binding Specificity: AA 65-275

Purpose: Anti-Mast Cell Tryptase/TPSAB1 Antibody Cy3 Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: coli-derived human Mast Cell Tryptase recombinant protein (Position: H65-P275). Human Mast Cell Tryptase shares 77%,76.1% amino acid (aa) sequence identity with mouse,rat Mast Cell Tryptase,respectively.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human Mast Cell Tryptase recombinant protein (Position: H65-P275). Human Mast Cell Tryptase shares 77% and 76.1% amino acid (aa) sequence identity with mouse and rat Mast Cell Tryptase, respectively.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for TPSAB1

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Alternative Name: TPSAB1

Background:

Background: Tryptase alpha-1 and tryptase beta-1 are enzymes that in humans are encoded by the same TPSAB1 gene. Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. In addition, these genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells, whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

Gene Full Name: tryptase alpha/beta 1

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Gene ID: 7177

UniProt: Q15661