UCHL3 antibody (AA 1-223)

Catalog No. ABIN8051985

The Rabbit Polyclonal anti-UCHL3 antibody is suitable to detect UCHL3 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IP, ICC and IF.

Quick Overview for UCHL3 antibody (AA 1-223) (ABIN8051985)

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCHL3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-UCHL3 Antibody

Binding Specificity: AA 1-223

Purpose: Anti-UCHL3 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human UCHL3 shares 98.2%,97.8% amino acid (aa) sequence identity with mouse,rat UCHL3,respectively.

Characteristics: Anti-UCHL3 Antibody. Tested in WB, ICC, IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UCHL3 recombinant protein (Position: M1-N223). Human UCHL3 shares 98.2% and 97.8% amino acid (aa) sequence identity with mouse and rat UCHL3, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UCHL3

Target: UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))

Alternative Name: UCHL3

Background:

Background: The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms.

Gene Full Name: ubiquitin C-terminal hydrolase L3

Molecular Weight: 26 kDa

Gene ID: 7347

UniProt: P15374

Pathways: Feeding Behaviour, Positive Regulation of fat Cell Differentiation