UFM1 antibody (AA 1-85)

Catalog No. ABIN8052440

This Rabbit Polyclonal antibody specifically detects UFM1 in IHC, WB, ELISA, FACS and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for UFM1 antibody (AA 1-85) (ABIN8052440)

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UFM1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-UFM1 Antibody

Binding Specificity: AA 1-85

Purpose: Anti-UFM1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: Human UFM1 shares 100%,98.8% amino acid (aa) sequence identity with mouse,rat UFM1,respectively.

Characteristics: Anti-UFM1 Antibody. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human UFM1 recombinant protein (Position: M1-C85). Human UFM1 shares 100% and 98.8% amino acid (aa) sequence identity with mouse and rat UFM1, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for UFM1

Target: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Alternative Name: UFM1

Background:

Background: UFM1 is a ubiquitin-like protein that is conjugated to target proteins by E1-like activating enzyme UBA5 (UBE1DC1, MIM 610552) and E2-like conjugating enzyme UFC1 (MIM 610554) in a manner analogous to ubiquitylation (see UBE2M, MIM 603173) (Komatsu et al., 2004 [PubMed 15071506]).

Gene Full Name: ubiquitin fold modifier 1

Molecular Weight: 10 kDa

Gene ID: 51569

UniProt: P61960