CrkL antibody (AA 1-290) (Cy3)

Catalog No. ABIN8052995

This Rabbit Polyclonal antibody specifically detects CrkL in WB, ELISA, IF, FACS, ICC and IP. It exhibits reactivity toward Human.

Quick Overview for CrkL antibody (AA 1-290) (Cy3) (ABIN8052995)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CrkL antibody is conjugated to Cy3

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Product Details anti-CrkL Antibody (Cy3)

Binding Specificity: AA 1-290

Purpose: Anti-CRKL Antibody Cy3 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CRKL recombinant protein (Position: M1-H290).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human ELISA, 0.1-0.5 μg/mL, -

Comment:

Applications are recommended based on the unconjugated antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CRKL

Background:

Background: Crk-like protein is a protein that in humans is encoded by the CRKL gene. This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.

Gene Full Name: CRK like proto-oncogene, adaptor protein

Gene ID: 1399

UniProt: P46109

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor