CrkL antibody (C-Term)

Catalog No. ABIN8053015

The Rabbit Polyclonal anti-CrkL antibody (ABIN8053015) specifically detects CrkL in WB and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CrkL antibody (C-Term) (ABIN8053015)

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CrkL antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: Carrier-free

Product Details anti-CrkL Antibody

Binding Specificity: C-Term

Purpose: Anti-Crk-like protein CrkL Antibody

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by one amino acid.

Characteristics: Anti-Crk-like protein CrkL Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Sodium azide, Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CrkL

Target: CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))

Alternative Name: CRKL

Background:

Background: CRKL (v-crk sarcoma virus CT10 oncogene homolog (avian)-like) also called ONCOGENE CRKL, encodes a 303-amino acid polypeptide with a predicted molecular mass of 36 kD. While CRKL is not the human homolog of CRK, it is similar to protein-tyrosine kinases with SH2 and SH3 (src homology) domains. By fluorescence in situ hybridization, the CRKL gene is localized to chromosome 22q11, centromeric of the chronic myelogenous leukemia breakpoint region. Senechal et al. (1996) showed that CRKL becomes phosphorylated when overexpressed, activates Ras-dependent and JNK pathways, and transforms fibroblasts. The authors also found CRKL to be a substrate for the BCR-ABL tyrosine kinase, leading them to conclude thatCRKL is a tyrosine kinase and an oncogene. Crkl was required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression.

Gene Full Name: CRK like proto-oncogene, adaptor protein

Sequence Similarities: Belongs to the CRK family.

Molecular Weight: 39 kDa

Gene ID: 1399

UniProt: P46109

Pathways: Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor