Versican antibody (N-Term) (FITC)

Catalog No. ABIN8053604

This Rabbit Polyclonal antibody specifically detects Versican in FACS. It exhibits reactivity toward Mouse, Human and Rat.

Quick Overview for Versican antibody (N-Term) (FITC) (ABIN8053604)

Target: Versican (Vcan)

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Versican antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-Versican Antibody (FITC)

Binding Specificity: N-Term

Purpose: Anti-Versican/VCAN Antibody FITC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins

Predicted Reactivity: different from the related mouse,rat sequences by two amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Versican, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for Versican

Target: Versican (Vcan)

Alternative Name: VCAN

Background:

Background: Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan with an apparent molecular mass of more than 1000 kDa. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.

Gene Full Name: versican

Sequence Similarities: Belongs to the aggrecan/versican proteoglycan family.

Gene ID: 1462

UniProt: P13611

Pathways: Glycosaminoglycan Metabolic Process