WISP1 antibody (AA 80-367) (Fluoro647)

Catalog No. ABIN8054445

The Rabbit Polyclonal anti-WISP1 antibody is suitable to detect WISP1 in samples from Human, Mouse and Rat. It has been validated for FACS.

Quick Overview for WISP1 antibody (AA 80-367) (Fluoro647) (ABIN8054445)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WISP1 antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-WISP1 Antibody (Fluoro647)

Binding Specificity: AA 80-367

Purpose: Anti-WISP-1/CCN4 Antibody Fluoro647 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human WISP-1/CCN4 recombinant protein (Position: A80-N367).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for WISP1

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Alternative Name: CCN4

Background:

Background: This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83 % identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified.

Gene Full Name: cellular communication network factor 4

Gene ID: 8840

UniProt: O95388

Pathways: WNT Signaling, Growth Factor Binding