XAF1 antibody (AA 197-301) (Fluoro647)

Catalog No. ABIN8054831

This Rabbit Polyclonal antibody specifically detects XAF1 in FACS. It exhibits reactivity toward Human.

Quick Overview for XAF1 antibody (AA 197-301) (Fluoro647) (ABIN8054831)

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This XAF1 antibody is conjugated to Fluoro647

Application: Flow Cytometry (FACS)

Product Details anti-XAF1 Antibody (Fluoro647)

Binding Specificity: AA 197-301

Purpose: Anti-XAF1 Antibody Fluoro647 Conjugated

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human XAF1 recombinant protein (Position: Q197-S301).

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for XAF1

Target: XAF1 (XiAP Associated Factor 1 (XAF1))

Alternative Name: XAF1

Background:

Background: XAF1 also called XIAP-associated factor 1,antagonizes the anticaspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. By genomic sequence analysis, the XAF1 gene contains 7 exons spanning 18 kb. Southern blot analysis suggested that XAF1 is a single-copy gene. The XAF1 gene is mapped on 17p13.1. Fluorescence microscopy demonstrated nuclear expression of XAF1, in contrast to the cytoplasmic expression of XIAP. Functional analysis indicated that XAF1 reverses the XIAP-mediated inhibition of CASP3 and its protection against apoptosis. Northern blot analysis detected wide expression of 3.9-, 4.5-, 6.0-, and 7.0-kb XAF1 transcripts, with highest levels in heart and ovary and lowest levels in brain and testis. Antisense experiments determined that loss of XAF1 expression enhances resistance to apoptosis. Alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Gene Full Name: XIAP associated factor 1

Gene ID: 54739

UniProt: Q6GPH4