ZEB1 antibody (Middle Region)

Catalog No. ABIN8055195

The Mouse Monoclonal anti-ZEB1 antibody is suitable to detect ZEB1 in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ICC.

Quick Overview for ZEB1 antibody (Middle Region) (ABIN8055195)

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Grade: Carrier-free

Clone: 8B12D7

Product Details anti-ZEB1 Antibody

Binding Specificity: Middle Region

Purpose: Anti-AREB6/ZEB1 Antibody (monoclonal, 8B12D7)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-AREB6/ZEB1 Antibody.(monoclonal, 8B12D7)-2. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human AREB6/ZEB1.

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1

Background:

Background: ZEB1 (Zinc Finger E Box-Binding Homeobox 1), also called TCF8, NIL2A or DELTA-EF1, is a protein that in humans is encoded by the ZEB1 gene. Fluorescence in situ hybridization localized the ZEB1 gene to chromosome 10p11.2. Krafchak et al. (2005) demonstrated a complex (core plus secondary) binding site for TCF8 in the promoter of the COL4A3 gene, mutant in Alport syndrome and which encodes collagen type IV alpha-3. They detected expression of TCF8 in cornea. Nishimura et al. (2006) found that delta-Ef1 was upregulated during differentiation in a mouse smooth muscle cell (SMC) line.

Gene Full Name: zinc finger E-box binding homeobox 1

Molecular Weight: 200 kDa

Gene ID: 6935

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation