GLI1 antibody (AA 800-1057) (Cy3)

Catalog No. ABIN8055274

The Cy3-conjugated Rabbit Polyclonal anti-GLI1 antibody (ABIN8055274) specifically detects GLI1 in FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GLI1 antibody (AA 800-1057) (Cy3) (ABIN8055274)

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLI1 antibody is conjugated to Cy3

Application: Flow Cytometry (FACS)

Product Details anti-GLI1 Antibody (Cy3)

Binding Specificity: AA 800-1057

Purpose: Anti-GLI1 Antibody Cy3 Conjugated

Predicted Reactivity: Human GLI1 shares 79.8% amino acid (aa) sequence identity with mouse GLI1.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GLI1 recombinant protein (Position: Y800-Q1057). Human GLI1 shares 79.8% amino acid (aa) sequence identity with mouse GLI1.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, 1-3 μg/1x10⁶ cells

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for GLI1

Target: GLI1 (Zinc Finger Protein GLI1 (GLI1))

Alternative Name: GLI1

Background:

Background: GLI1, also known as GLI, is a protein originally isolated in human glioblastoma. The protein is encoded by GLI1 gene. It is mapped to 12q13.3. This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Beside, various upstream patterning signals may be integrated by the Gli proteins to a coherent programme of neurogenesis.

Gene Full Name: GLI family zinc finger 1

Gene ID: 2735

UniProt: P08151

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis