ZP2 antibody (N-Term) (APC)

Catalog No. ABIN8055357

The Rabbit Polyclonal anti-ZP2 antibody is suitable to detect ZP2 in samples from Human. It has been validated for FACS.

Quick Overview for ZP2 antibody (N-Term) (APC) (ABIN8055357)

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZP2 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Product Details anti-ZP2 Antibody (APC)

Binding Specificity: N-Term

Purpose: Anti-ZP2 Antibody APC Conjugated

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: different from the related mouse sequence by six amino acids,and from the related rat sequence by four amino acids.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ZP2, different from the related mouse sequence by six amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Flow Cytometry, Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Each vial contains 50 % glycerol, 0.9 % NaCl, 0.2 % Na2HPO4, 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.

Expiry Date: 12 months

Target Details for ZP2

Target: ZP2 (Zona Pellucida Glycoprotein 2 (ZP2))

Alternative Name: ZP2

Background:

Background: Zona pellucida sperm-binding protein 2 is a protein that in humans is encoded by the ZP2 gene. The sperm-binding domain on the ZP2 protein is necessary in both humans and mice for oocyte-sperm recognition and penetration of the zona pellucida. It is also responsible for the primary block to polyspermy in mammals. The oocyte has cortical granules peripherally located under the cortex that contain a proteolytic protein called ovastacin. After the sperm binds to ZP2, the cortical granules are exocytosed releasing ovastacin into the perivitelline space. Ovastacin cleaves ZP2 at the N terminus, preventing more sperm from binding and penetrating the oocyte, thus hardening the zona pellucida. Ovastacin is only found in oocytes, and is part of the astacin family of metalloendoproteases. Female mice engineered without ovastacin showed that ZP2 was not cleaved after fertilization.

Gene Full Name: zona pellucida glycoprotein 2

Gene ID: 7783

UniProt: Q05996