Recombinant ARL13B antibody (C-Term) (FL550)

Catalog No. ABIN8065417

The FL550-conjugated Guinea Pig Chimeric anti-ARL13B antibody (Clone N295B-66) (ABIN8065417) specifically detects ARL13B in IHC and ICC. The antibody is reactive with Mouse samples.

Quick Overview for Recombinant ARL13B antibody (C-Term) (FL550) (ABIN8065417)

Target: ARL13B (ADP-Ribosylation Factor-Like 13B (ARL13B))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Guinea Pig, Mouse

Clonality: Chimeric

Conjugate: This ARL13B antibody is conjugated to FL550

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Grade: KO Validated

Clone: N295B-66

Product Details anti-ARL13B Antibody (FL550)

Binding Specificity: AA 208-427, C-Term

Expression System: Mammalian cells

Purpose: Anti-Arl13b (N295B/66) Recombinant Guinea Pig Chimeric mAb FL550 Conjugate

Specificity: Does not cross-react with Arl13a (based on KO validation results)

Predicted Reactivity: Human,Mouse,Non-Human Primate,Rat,and Zebrafish

Characteristics: This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N295B/66 with guinea pig IgG heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N295B/66 but can be detected using standard anti-guinea pig secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and conjugated.

Purification: Purified by affinity chromatography

Immunogen: Fusion protein amino acids 208-427 (C-terminus) of mouse Arl13b (accession number Q640N2) produced recombinantly in E. Coli

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 1X PBS, 0.05 % Sodium Azide pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 24 months

Target Details for ARL13B

Target: ARL13B (ADP-Ribosylation Factor-Like 13B (ARL13B))

Alternative Name: Arl13b

Background: ADP-ribosylation factor-like protein 13B (ADP-ribosylation factor-like protein 2-like 1) (ARL2-like protein 1) (Protein hennin),Arl13b Arl2l1,ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1. Arl13B is a small GTPase with both N-terminal and C-terminal guanine nucleotide-binding motifs. Arl13B is localized to the membrane of cilia and expressed in Neuroepithelial cells and developing radial glia of the developing cerebral cortex. Mutations in this gene are the cause of Joubert syndrome 8.

Molecular Weight: 50 kDa

UniProt: Q640N2