Fibronectin antibody

Catalog No. ABIN8065460

This Mouse Monoclonal antibody specifically detects Fibronectin in WB, IHC, ELISA and IP. It exhibits reactivity toward Cow.

Quick Overview for Fibronectin antibody (ABIN8065460)

Target: Fibronectin (FN)

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Grade: Carrier-free

Clone: A32

Product Details anti-Fibronectin Antibody

Purpose: Anti-Fibronectin Antibody

Specificity: CSI 005-32 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). The antibody inhibits cell adhesion to fibronectin mediated by the heparin-binding domain. Epitope is located in the 40kD Hep II heparin-binding domain.

Predicted Reactivity: Bovine,Human

Purification: Protein A or Protein G purified

Immunogen: Bovine corneal endothelial cells

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C without exposure to light

Expiry Date: 24 months

Target Details for Fibronectin

Target: Fibronectin (FN)

Alternative Name: Fibronectin

Background: FN1, fibronectin 1,Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). {ECO:0000250|UniProtKB:P02751, ECO:0000250|UniProtKB:P11276}., [Anastellin]: Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. {ECO:0000250|UniProtKB:P02751}., Secreted by contracting muscle, induces liver autophagy, a degradative pathway for nutrient mobilization and damage removal, and systemic insulin sensitization via hepatic ITGA5:ITGB1 integrin receptor signaling. {ECO:0000250|UniProtKB:P11276}.,FN1,Fibronectin is an adhesive glycoprotein with a molecular mass of 440 kDa. It is believed to be important for the formation of a provisional matrix that promotes cell adhesion and migration during wound healing. Its age-dependent increase in plasma and tissues may be accompanied in pathological states, especially in tumor growth, by its proteolytic breakdown by a number of neutral proteases. It has also shown that several of its proteolytic breakdown products exhibit unexpected and mostly harmful biological activities. (1)

Molecular Weight: 440 kDa glycoprotein dimer, with monomers running at 260 on SDS-PAGE

Gene ID: 280794

UniProt: P07589