Ficolin 2 antibody

Catalog No. ABIN8065462

The Mouse Monoclonal anti-Ficolin 2 antibody (Clone 19) (ABIN8065462) specifically detects Ficolin 2 in WB, IP and ELISA. The antibody is reactive with Human samples.

Quick Overview for Ficolin 2 antibody (ABIN8065462)

Target: Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ficolin 2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Grade: Carrier-free

Clone: 19

Product Details anti-Ficolin 2 Antibody

Purpose: Anti-L-Ficolin Antibody

Specificity: ABS 005-19 reacts specifically with L-Ficolin. No cross reactivity is seen with M-Ficolin (Ficolin-1) and H-Ficolin (Ficolin-3).

Predicted Reactivity: Human

Purification: Protein A or Protein G purified

Immunogen: Purified procaryot expressed recombinant human L-Ficolin and full-length L-Ficolin (Ficolin-2) protein.

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2-8°C without exposure to light

Expiry Date: 24 months

Target Details for Ficolin 2

Target: Ficolin 2 (FCN2) (Ficolin (Collagen/fibrinogen Domain Containing Lectin) 2 (Hucolin) (FCN2))

Alternative Name: L-Ficolin

Background: FCN2, EBP-37, FCNL, P35, ficolin-2, ficolin-2, 37 kDa elastin-binding protein, L-ficolin, collagen/fibrinogen domain-containing protein 2, ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin), ficolin B, ficolin-beta, serum lectin p35,Calcium-dependent lectin, which acts as a pattern recognition receptor that initiates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed:10679061, PubMed:14707097, PubMed:15804047, PubMed:17215869, PubMed:25344472, PubMed:8576206). Specifically recognizes and binds carbohydrates on the pathogen surface, activating the MASP1 serine protease and initiating the proteolytic cascade of the lectin complement pathway (PubMed:14707097). Specifically binds N-Acetylglucosamine (GlcNAc), as well as phosphocholine and lipoteichoic acid moieties on the surface of pathogens (PubMed:14707097, PubMed:17215869, PubMed:25344472, PubMed:8576206). Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect (PubMed:8576206). {ECO:0000269|PubMed:10679061, ECO:0000269|PubMed:14707097, ECO:0000269|PubMed:15804047, ECO:0000269|PubMed:17215869, ECO:0000269|PubMed:25344472, ECO:0000269|PubMed:8576206}.,FCN2,L-Ficolin (Ficolin-2) is an innate immunity pattern recognition molecule found in human serum. L-Ficolin binds to distinct pathogen-associated molecular patterns (PAMP), such as carbohydrates (GlcNAc), lipoteichoic acid and acetylated groups thereby facilitating phagocytosis and activation of complement through the lectin route using the same serine proteases as mannose-binding lectin (MBL) named MASP ((MBL)-associated serine protease) (1).

Molecular Weight: 35 kDa

Gene ID: 2220

UniProt: Q15485

Pathways: Complement System